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Related Experiment Video

Updated: Oct 31, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

miniSPIM-A Miniaturized Light-Sheet Microscope.

Per Niklas Hedde^1,2,3

¹Beckman Laser Institute, University of California Irvine, Irvine, California 92612, United States.

|July 1, 2021

Summary

This summary is machine-generated.

The miniSPIM (miniature scanning probe microscopy) is a portable light-sheet microscope for mobile devices. It enables optical sectioning in diverse applications like biosensing and field research.

Keywords:

bacteria detection fluorescence fluctuation spectroscopy image correlation spectroscopy light-sheet microscopy optical sectioning particle detection selective plane illumination microscopy three-dimensional imaging

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Last Updated: Oct 31, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

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Published on: May 5, 2014

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

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Published on: January 16, 2018

Area of Science:

Microscopy
Biophotonics
Optical Engineering

Background:

Traditional light-sheet microscopes are often bulky and power-intensive.
There is a need for portable, low-cost imaging solutions for field and educational use.

Purpose of the Study:

To develop a miniaturized light-sheet microscope (miniSPIM) integrated into a small footprint.
To demonstrate the versatility of the miniSPIM for various scientific applications.

Main Methods:

Integration of light-sheet microscopy components into a smartphone-sized device.
Utilizing mobile camera devices and single-board computers for imaging.
Demonstrating applications including particle tracking, spectral analysis, and 3D imaging of biological samples.

Main Results:

Successful miniaturization of a light-sheet microscope within a compact form factor.
Quantification of microparticle and bacteria motion in fluids.
Characterization of solvent polarity using Nile Red spectral shifts.
3D and time-lapse imaging of a zebrafish embryo's autofluorescence.

Conclusions:

The miniSPIM offers a portable, robust, and cost-effective solution for optical sectioning microscopy.
Its applications span biosensing, field research, and educational settings.
The technology enables advanced imaging capabilities on mobile platforms.