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Isocratic reporter-exclusion immunoassay using restricted-access adsorbents
Sagar Dhamane1, Ujwal Patil1, Maxwell Smith2
1Department of Biology and Biochemistry, University of Houston, Houston, TX, USA. willson@uh.edu.
The Analyst
|July 1, 2021
Summary
A novel immunoassay uses reporter exclusion from adsorbents to detect analytes like viruses and proteins. This method offers a rapid, cost-effective alternative to traditional immunoassays, utilizing broadly applicable column materials.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Background:
- Immunoassays are crucial for detecting various analytes but often rely on costly, specific materials.
- Current methods can be time-consuming and require specialized reagents.
Purpose of the Study:
- To develop a novel, cost-effective immunoassay method based on reporter exclusion.
- To demonstrate the applicability of this method for detecting diverse analytes, including viruses and proteins.
Main Methods:
- Utilized restricted-access adsorbents with a size-selective outer layer and a mixed-mode capture core.
- Developed an isocratic reporter-exclusion immunoassay principle where analyte presence dictates reporter reagent capture.
- Demonstrated the assay using M13 bacteriophage virus and human chorionic gonadotropin as model analytes.
Main Results:
- Reporter reagents were excluded from adsorbents when bound to analytes, enabling signal detection.
- Analyte-dependent exclusion allowed for the differentiation of analyte presence or absence.
- Successful detection of model analytes (M13 bacteriophage and human chorionic gonadotropin) was achieved.
Conclusions:
- The reporter-exclusion immunoassay offers a simple, rapid, and cost-effective approach for analyte detection.
- This method utilizes inexpensive, stable, and generally applicable column materials, avoiding analyte-specific immunoaffinity adsorbents.
- The principle is broadly applicable for detecting a wide range of analytes, including viruses and proteins.
