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The Unusual Role of Pro in Cu(II) Binding by His₂-Cyclopentapeptide

Aleksandra Pieniężna¹, Aleksandra Kotynia¹, Justyna Brasuń¹

¹Department of Inorganic Chemistry, Wroclaw Medical University, Borowska 211A, 50-552 Wrocław, Poland.

International Journal of Molecular Sciences

|July 2, 2021

Abstract:

In this paper, we present findings from studying the interaction of copper(II) ions with the His₂-cyclopentapeptide and the role of proline used for the purpose of potentiometric titration and UV-Vis, CD and EPR spectroscopic measurements. Experiments of two homodetic peptides differing by one amino acid residue were conducted for a ligand to metal ratio of 1:1 in the pH range 2.5-11.0. The presented studies reveal that peptides form only mononuclear complexes, and the CuH₂L complex appears in the system first (for both L1 and L2). Study results show that the presence of Pro influences the structure of formed complexes and their stabilities and has a strong impact on the efficiency of copper(II) coordination.

Keywords:

coordination copper(II) ions cyclopeptides potentiometric measurements proline spectroscopy

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