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Updated: Oct 30, 2025

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

High-Throughput Cell Concentration Using A Piezoelectric Pump in Closed-Loop Viscoelastic Microfluidics.

Jeeyong Kim¹, Hyunjung Lim^2,3, Hyunseul Jee^3,4

¹Department of Laboratory Medicine, College of Medicine, Korea University, Seoul 08307, Korea.

|July 2, 2021

Summary

A novel closed-loop system effectively concentrates rare cells using viscoelastic fluids and piezoelectric pumps. This high-throughput method achieves significant cell concentration for improved biological assays and diagnostics.

Keywords:

closed-loop concentration high-throughput piezoelectric pump viscoelastic fluid

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Last Updated: Oct 30, 2025

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Area of Science:

Biotechnology
Microfluidics
Cellular Biology

Background:

Conventional cell concentration methods like centrifugation are batch-based, slow, and require bulky equipment.
Achieving high concentration ratios in a single pass for rare cell detection is challenging.
There is a need for high-throughput, continuous cell concentration techniques for diagnostics.

Purpose of the Study:

To develop a closed-loop continuous cell concentration system utilizing viscoelastic non-Newtonian fluids.
To miniaturize and integrate the system using piezoelectric pumps for high-throughput applications.
To evaluate the system's performance in concentrating various cell types from different biological samples.

Main Methods:

A closed-loop microfluidic system was designed incorporating two piezoelectric pumps.
The system employed a viscoelastic non-Newtonian fluid to facilitate cell focusing.
Concentration performance was assessed using 13 μm particles, white blood cells (WBCs), and cancer cells in blood and urine samples.

Main Results:

The closed-loop system achieved significant concentration factors for WBCs (~63.4-fold) and prostate cancer cells (~64.1-fold).
High hematocrit (70%) lysed blood samples and urine samples were successfully processed.
Concentration was achieved within short timeframes (20 min for blood, 11 min for urine) with reduced final volumes.

Conclusions:

The developed closed-loop system offers a high-throughput, continuous method for efficient cell concentration.
This technique overcomes limitations of conventional methods, enabling better pre-treatment for rare cell detection.
The system demonstrates clinical applicability for concentrating cells from complex biological matrices like blood and urine.