Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-step yielding in a jammed microgel suspension.

Soft matter·2026
Same author

Assessment of pH-Responsive Ionisable Lipid Nanoparticles as Cisplatin Delivery Vehicles for Treating Cisplatin-Resistant Ovarian Cancer.

Pharmaceutics·2026
Same author

Aptamer-Loaded Cubosome Lipid Nanoparticles for the Treatment of Tuberculosis.

ACS applied materials & interfaces·2026
Same author

Unexpected Inner-Sphere Versus Outer-Sphere Redox in Bilayer Molybdenum Disulfide (MoS<sub>2</sub>) from Correlative Electrochemical Imaging.

Chemical & biomedical imaging·2026
Same author

Stem cell preservation with novel cryoprotectants.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same author

Correction to "Phase Characterization and Bioactivity Evaluation of Nucleic Acid-Encapsulated Biomimetically Mineralized ZIF-8".

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: Oct 30, 2025

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

31.6K

Cryopreservation of mammalian cells using protic ionic liquid solutions.

Saffron J Bryant1, Stuart J Brown1, Andrew V Martin1

  • 1School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, Australia.

Journal of Colloid and Interface Science
|July 2, 2021
PubMed
Summary

Ionic liquids show promise as less-toxic cryoprotective agents. Ethylammonium acetate effectively cryopreserved a mammalian cell line, matching dimethylsulfoxide efficacy.

Keywords:
BiomedicineCellCryopreservationCryoprotetive agentsIonic liquidsLong term preservationNanomedicineProtic ionic liquidsStorage

More Related Videos

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.0K
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
06:42

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

3.6K

Related Experiment Videos

Last Updated: Oct 30, 2025

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

31.6K
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.0K
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
06:42

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

3.6K

Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Cryopreservation advances are hindered by limited effective and non-toxic cryoprotective agents.
  • Current protocols predominantly use toxic agents like dimethylsulfoxide (DMSO) and glycerol.
  • Ionic liquids (ILs) are explored as potential alternatives due to their glass-forming properties.

Purpose of the Study:

  • To investigate protic ionic liquids (PILs) as novel cryoprotective agents.
  • To screen ten PILs for cellular toxicity, permeability, and thermal behavior.
  • To evaluate the efficacy of the most promising PIL in cryopreserving a model mammalian cell line.

Main Methods:

  • Screening of ten protic ionic liquids for cryoprotective properties.
  • Assessment of cellular toxicity, permeability, and thermal characteristics.
  • Cryopreservation efficacy testing of ethylammonium acetate on a model cell line.

Main Results:

  • Ethylammonium acetate demonstrated comparable efficacy to dimethylsulfoxide in cryopreserving a mammalian cell line.
  • The study identified ethylammonium acetate as a promising cryoprotective agent.
  • This research marks the first successful use of a protic ionic liquid for mammalian cell cryopreservation.

Conclusions:

  • Protic ionic liquids, specifically ethylammonium acetate, represent a viable, less-toxic alternative to traditional cryoprotectants.
  • This finding opens avenues for developing new IL-based cryoprotectants for a wider range of cell types.
  • Further development of ILs could significantly advance cryopreservation technologies in medicine and research.