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Persistent luminescence nanorods-based autofluorescence-free biosensor for prostate-specific antigen detection
Zipeng Yin1, Ling Zhu1, Zijian Lv1
1Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Fujian Provincial Key Laboratory of Electrochemical Energy Storage Materials, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University, Fuzhou, 350108, PR China.
Talanta
|July 3, 2021
Summary
Persistent luminescent nanoparticles (PLNPs) offer autofluorescence-free detection. This study developed a sensitive biosensor using ZGOMP nanoparticles for prostate-specific antigen (PSA) detection.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Persistent luminescent nanoparticles (PLNPs) emit light after excitation removal, enabling autofluorescence-free biological detection.
- Developing sensitive and selective biosensors is crucial for early disease diagnosis.
Purpose of the Study:
- To develop an autofluorescence-free biosensor for prostate-specific antigen (PSA) detection.
- To utilize the pH-responsive luminescence of Zn2GeO4: Mn2+, Pr3+ (ZGOMP) nanorods for sensing applications.
Main Methods:
- Preparation of ZGOMP nanorods via a one-pot hydrothermal method.
- Design of a biosensor using ZGOMP as a probe and gluconic acid as a quencher.
- Incorporation of hybridization chain reaction (HCR) and magnetic separation for signal amplification.
Main Results:
- The ZGOMP-based biosensor demonstrated pH-responsive luminescence.
- High selectivity and sensitivity for PSA detection were achieved.
- A wide linear range (0.001-10 ng/mL) and a low detection limit (0.64 pg/mL) were obtained.
Conclusions:
- The developed autofluorescence-free sensing platform shows excellent analytical performance for PSA detection.
- This strategy provides an effective platform for clinical sample analysis.
- The pH-responsive ZGOMP nanorods are promising for developing advanced biosensing applications.
