[Microbial synthesis of plant polyphenols]

Area of Science:

• Biotechnology
• Synthetic Biology
• Metabolic Engineering

Background:

• Plant polyphenols, including phenolic acids, stilbenes, curcumins, and flavonoids, possess diverse biological activities and significant application potential in pharmaceuticals, food, cosmetics, and chemicals.
• Microorganisms offer advantages for heterologous natural product synthesis, including rapid growth, ease of culture, and industrial scalability.

Purpose of the Study:

• To review the microbial synthesis of plant polyphenols using engineered microorganisms.
• To discuss strategies for optimizing polyphenol production in engineered microbes.
• To explore future prospects in polyphenol pathway engineering.

Main Methods:

• Engineering of microorganisms such as Escherichia coli and Saccharomyces cerevisiae with designed biosynthetic pathways for polyphenol production.
• Application of synthetic biology tools to construct and optimize metabolic pathways.
• Implementation of optimization strategies including precursor engineering, dynamic pathway regulation, and co-cultivation.

Main Results:

• Substantial progress has been achieved in the microbial synthesis of various plant polyphenols.
• Engineered microorganisms can be equipped with designed biosynthetic pathways for efficient polyphenol production.
• Optimization strategies have been identified to enhance polyphenol yields.

Conclusions:

• Microbial synthesis represents a promising approach for sustainable and scalable production of plant polyphenols.
• Further advancements in synthetic biology and metabolic engineering will continue to improve polyphenol yields and expand their applications.
• Pathway engineering in microorganisms holds significant future potential for natural product manufacturing.