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Updated: Oct 29, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Published on: September 2, 2021
Optical Control of Genome Editing by Photoactivatable Cas9
Takahiro Otabe1, Yuta Nihongaki2, Moritoshi Sato3
1Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan.
Abstract:
The CRISPR-Cas9 system offers targeted genome manipulation with simplicity. Combining the CRISPR-Cas9 with optogenetics technology, we have engineered photoactivatable Cas9 to precisely control the genome sequence in a spatiotemporal manner. Here we provide a detailed protocol for optogenetic genome editing experiments using photoactivatable Cas9, including that for the generation of guide RNA vectors, light-mediated Cas9 activation, and quantification of genome editing efficiency in mammalian cells.
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