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Updated: Oct 29, 2025

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
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Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme

Yasunori Okamoto1, Ryosuke Kojima2,3

  • 1Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan. yasunori.okamoto@tohoku.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|July 6, 2021
PubMed

Keywords:
Allylic dealkylationArtificial metalloenzymeCell-penetrating polydisulfidesGene switchIntracellular catalysisRuthenium complexStreptavidinThyroid hormone

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