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Related Experiment Video

Updated: Oct 29, 2025

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Magnetically Single-Cell Virus Stamping.

Rajib Schubert¹

¹Research and Early Development, Roche Molecular Systems, Pleasanton, CA, USA. rac0820@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|July 6, 2021

Summary

This summary is machine-generated.

This study introduces a precise method for targeted virus infection of single cells, enabling better understanding of complex tissues. The technique achieves high success rates for stable genetic modification in just minutes.

Keywords:

Animal cell Cultured neuron Electromagnet Enveloped virus Lentivirus Magnetic guidance Magnetic nanoparticle Optical guidance Single cell transduction Virus stamping Virus transduction

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Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Viral transduction is crucial for genetic engineering of single cells to study complex tissues.
Current viral delivery methods have limitations hindering precise single-cell genetic manipulation.
Optically accessible cell monolayers are ideal for developing targeted infection protocols.

Purpose of the Study:

To present a precise protocol for targeted virus infection of single cells.
To overcome limitations of current viral delivery methods in genetic engineering.
To enable efficient and stable transgene expression in single cells.

Main Methods:

Developed a protocol for precise, targeted virus infection of single cells in optically accessible monolayers.
Utilized lentiviruses (LVs) for genetic manipulation.
Demonstrated the technique by stamping cultured Hela cells with LVs.

Main Results:

The protocol allows targeted virus infection of single cells in minutes.
Achieved stable transgene expression within a few days post-infection.
Reported success rates approaching 80% for targeted single-cell infection.

Conclusions:

The presented protocol offers a precise and efficient method for single-cell genetic engineering.
This technique enhances the understanding of complex tissues through reliable viral transduction.
The high success rate and speed make this protocol valuable for various research applications.