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Updated: Oct 29, 2025
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Structure-selective differentiation of deletion mutations in circulating tumor DNA using dual probe-based isothermal
Yusong Wang1, Say Li Kong2, Xiaodi Su3
1Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 2 Fusionopolis Way. Innovis, #8-03, 138634, Singapore. Wang_Yusong@ibb.a-star.edu.sg xd-su@imre.a-star.edu.sg.
Abstract:
A rapid dual probe-based fluorimetric assay was developed to detect deletion mutations in circulating tumor DNA using structure-selective isothermal amplification and pattern recognition. This method could detect both homozygous and heterozygous deletion configurations in a one-set experiment and achieved picomolar detection limits with high selectivity within 2 hours. It was promising for point-of-care cancer diagnosis in hospital settings.
