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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Updated: Oct 29, 2025

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Published on: October 25, 2019

Bioinspired materials and technology for advanced cryopreservation.

Mengjia Dou¹, Chennan Lu², Wei Rao²

¹Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; School of Engineering Science, University of Chinese Academy of Sciences, Beijing, 100049, China; Beijing Key Laboratory of Cryo-Biomedical Engineering, Beijing, 100190, China.

Trends in Biotechnology

|July 9, 2021

Summary

This summary is machine-generated.

Nature provides models for advanced cryopreservation. Studying hibernators and insects can lead to better methods for preserving sensitive cells, tissues, and organs for medical use.

Keywords:

cryoprotectants freeze-tolerant hibernation microfluidic technology nanotechnology supercooling

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Area of Science:

Cryobiology
Biomedical Engineering
Zoology

Background:

Cryopreservation is crucial for medical biosamples, but challenges remain for sensitive cells, tissues, and organs.
A holistic approach to cryopreservation is needed to improve its efficacy.

Purpose of the Study:

To explore natural models for advancing cryopreservation techniques.
To investigate the anti-cold strategies of cold-tolerant organisms for potential applications in cryoprotection.

Main Methods:

Studying the biological mechanisms of animals that survive in cold temperatures, such as metabolic suppression and cryoprotectant synthesis.
Investigating the discovery and synthesis of novel biomaterials for metabolism regulation and cryoprotection.

Main Results:

Animals like hibernators and cold-tolerant insects possess natural strategies for surviving extreme cold.
These strategies include programmed metabolic suppression and the endogenous synthesis of cryoprotectants (CPAs).

Conclusions:

Understanding natural cryoprotection mechanisms can inspire new cryopreservation technologies.
Biomaterials and biotechnological advancements, informed by nature, can enhance biosample storage quality and duration for clinical applications.