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Related Concept Videos

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Related Experiment Video

Updated: Oct 29, 2025

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
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A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

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Germ cells: ENCODE's forgotten cell type†.

John R McCarrey1, Keren Cheng1

  • 1Department of Biology, University of Texas at San Antonio, San Antonio, TX USA.

Biology of Reproduction
|July 12, 2021
PubMed
Summary
This summary is machine-generated.

Epigenomic profiling data is lacking for male and female germ cells. This study highlights the need for such data and presents new epigenomic profiles for mammalian spermatogonial stem cells.

Keywords:
cell fatechromatinepigenetic profilingprogenitor spermatogoniaspermatogonial stem cells

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Area of Science:

  • Genomics
  • Epigenetics
  • Reproductive Biology

Background:

  • Large-scale epigenomic profiling has been performed for over 110 mammalian somatic cell types by the ENCODE and NIH Epigenomics Roadmap consortia.
  • These consortia generated valuable, publicly accessible datasets enabling genome-wide analysis of epigenetic parameters.
  • However, germ cell types were excluded, leaving a gap in comprehensive epigenetic data for these crucial cells.

Purpose of the Study:

  • To address the lack of comprehensive epigenomic data for mammalian germ cells.
  • To emphasize the importance of generating multiparametric epigenomic datasets for male and female germ cells at various developmental stages.
  • To present initial epigenomic profiling data for mammalian spermatogonial stem cells and progenitor spermatogonia.

Main Methods:

  • Discusses the necessity and approach for generating multiparametric epigenomic profiling datasets.
  • Summarizes a recent effort to derive such data for mammalian spermatogonial stem cells and progenitor spermatogonia.
  • Leverages established consortia approaches for epigenomic data generation and analysis.

Main Results:

  • Identifies a significant gap in publicly available epigenomic data for male and female germ cells.
  • Highlights the evolutionary importance of germ cells and the need for their epigenetic characterization.
  • Presents preliminary epigenomic profiling data for specific male germ cell populations.

Conclusions:

  • Comprehensive epigenomic profiling of germ cells is essential for understanding reproductive biology and evolution.
  • There is a critical need for reproductive biologists to generate and share such data.
  • The presented work is a foundational step towards filling this knowledge gap.