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Updated: Oct 28, 2025

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Processing technology optimization for tofu curded by fermented yellow whey using response surface methodology.

Zhanrui Huang1, Wanying He1, Liangzhong Zhao1

  • 1College of Food and Chemical Engineering Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control Shaoyang University Shaoyang China.

Food Science & Nutrition
|July 15, 2021
PubMed
Summary

Optimizing fermented yellow whey (FYW) content and processing parameters significantly enhances tofu yield and protein content. This research provides a basis for standardized, high-quality tofu production.

Keywords:
boiling‐to‐filtering methodfermented yellow wheyprocess optimizationsoybean producttofu

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Area of Science:

  • Food Science and Technology
  • Biotechnology
  • Protein Chemistry

Background:

  • Tofu production involves complex technological applications affecting yield and quality.
  • Soybean protein coagulation using fermented yellow whey (FYW) is explored for tofu production.
  • Optimizing processing parameters is crucial for maximizing tofu yield and protein content.

Purpose of the Study:

  • To investigate the gel-forming principle of soybean protein coagulated with FYW.
  • To optimize processing parameters for tofu production using the boiling-to-filtering method (BFM).
  • To enhance tofu yield and protein content through parameter optimization.

Main Methods:

  • Response surface methodology was employed to study and optimize processing parameters.
  • Key parameters investigated include soybean-to-water ratio, boiling temperature, boiling time, and FYW content.
  • The boiling-to-filtering method (BFM) was utilized for tofu production.

Main Results:

  • FYW content was identified as the most significant factor influencing tofu yield and protein content (p < .05).
  • Optimal BFM parameters were determined as: 1:5 soybean-to-water ratio, 6.1 min boiling time, 105°C boiling temperature, and 26% FYW content.
  • Optimized conditions resulted in a tofu yield of 235.17 g/100 g and protein content of 10.60%.

Conclusions:

  • Optimized processing parameters significantly increased tofu yield and protein content compared to unoptimized conditions.
  • The study provides practical technical support for the industrial production of high-yield, high-protein tofu.
  • This research lays a theoretical foundation for standardized tofu manufacturing using FYW.