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Updated: Oct 28, 2025

A Within-Subject Experimental Design using an Object Location Task in Rats
Published on: May 6, 2021
Case Study 1: Practical Considerations with Experimental Design and Interpretation
John T Barr1, Otito F Iwuchukwu2
1Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck, South San Francisco, CA, USA.
Abstract:
This chapter deals with practical considerations on key issues such as choosing an enzyme source, determining linear conditions, and choosing appropriate substrate and organic solvent concentrations. Practical solutions for working with limited resources and carrying out inhibition experiments are also addressed. Thus, after reading this chapter, the novice reader should have a better idea of how to design, develop, and interpret basic experiments using drug metabolism enzymes.
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