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Updated: Oct 28, 2025
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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
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Oocyte specific lncRNA variant Rose influences oocyte and embryo development
Rajan Iyyappan1, Daria Aleshkina1, Linkai Zhu2
1Laboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic.
Non-Coding RNA Research
|July 19, 2021
Summary
We identified a novel long non-coding RNA (lncRNA), named "Rose", crucial for mammalian oocyte and embryo development. Knocking down Rose disrupted oocyte cytokinesis and impaired embryo development.
Area of Science:
- Reproductive Biology
- Molecular Biology
- Genetics
Background:
- Mammalian oocytes accumulate significant RNA during growth, including long non-coding RNAs (lncRNAs).
- The precise roles of lncRNAs in oocyte physiology are not well understood.
- Identifying specific lncRNAs is key to understanding their cellular functions.
Purpose of the Study:
- To identify and characterize oocyte-specific lncRNAs.
- To investigate the functional significance of the identified lncRNA in oocyte and embryo development.
Main Methods:
- Identification and characterization of a novel oocyte-specific antisense lncRNA, "Rose" (lncRNA in Oocyte Specifically Expressed).
- Analysis of Rose expression variants and subcellular localization.
- Experimental manipulation (overexpression and knockdown) of Rose in oocytes.
- Assessment of meiotic maturation, oocyte cytokinesis, and preimplantation embryo development.
Main Results:
- "Rose" lncRNA was identified with two variants, localized to the nucleus in oocytes and the cytoplasm in embryos.
- Overexpression of Rose did not affect meiotic maturation.
- Knockdown of Rose led to abnormal oocyte cytokinesis and compromised preimplantation embryo development.
Conclusions:
- "Rose" is an oocyte-specific maternal lncRNA essential for mammalian oocyte and embryo development.
- This finding highlights the critical role of specific lncRNAs in reproductive processes.
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