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Updated: Oct 27, 2025

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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A [3Fe-4S] cluster and tRNA-dependent aminoacyltransferase BlsK in the biosynthesis of Blasticidin S

Xiankun Wang1,2,3, Yuchun Zhao1, Yaojie Gao1

  • 1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Proceedings of the National Academy of Sciences of the United States of America
|July 20, 2021
PubMed

Keywords:
iron–sulfur clusterleucyl transfer reactionnatural producttRNA-dependent enzymes

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