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Characterization of Paramagnetic Iron-Sulfur Clusters Using Electron Paramagnetic Resonance Spectroscopy

Troy A Stich1

  • 1Department of Chemistry, Wake Forest University, Winston-Salem, NC, USA. stichta@wfu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 22, 2021
PubMed
Summary

Continuous-wave electron paramagnetic resonance (EPR) spectroscopy identifies paramagnetic iron-sulfur ([Fe-S]) clusters in biological samples. This technique analyzes EPR spectra to reveal the electronic structure and oxidation states of these crucial [Fe-S] proteins.

Keywords:
CW-EPREPRESRElectron paramagnetic resonanceElectron transferFerredoxinIron-sulfurNitrogenaseRadical SAMRieske[Fe-S]continuous-wave EPR

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