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Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

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A specific and bioactive polysaccharide marker for Cordyceps.

Li-Feng Li¹, Grace Wing-Chiu But², Quan-Wei Zhang³

¹School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China; Hong Kong Authentication Centre of Valuable Chinese Medicines, Hong Kong, China.

Carbohydrate Polymers

|July 23, 2021

Summary

Researchers identified a unique polysaccharide fraction (CCP) specific to Cordyceps caterpillar parts. This bioactive marker enables both quality and quantity assessment, addressing authenticity concerns for this valuable functional food.

Keywords:

Authentication Cordyceps Immune activity Marker Polysaccharide

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Area of Science:

Mycology
Biochemistry
Immunology

Background:

Cordyceps is a highly valued functional food, but its authenticity is often questionable.
Lack of reliable markers hinders accurate identification and quality control of Cordyceps products.

Purpose of the Study:

To develop a specific and bioactive marker for authenticating Cordyceps.
To identify a polysaccharide marker for both qualitative and quantitative analysis.

Main Methods:

Screening of 250 samples using genetic markers and polysaccharide profiling.
Characterization of the unique polysaccharide fraction (CCP) using structural analysis and oligosaccharide sequencing.
Assessing the bioactivity of CCP by measuring nitric oxide (NO) and cytokine production in RAW 264.7 cells.

Main Results:

A unique polysaccharide fraction, CCP, was identified and found exclusively in the caterpillar parts of Cordyceps.
CCP demonstrated bioactivity by inducing NO and cytokine production.
Structural analysis revealed CCP as an α-1,4-glucan with a C-6 branch.
CCP content correlated with traditional classification criteria, validating its use as a marker.

Conclusions:

The polysaccharide fraction CCP serves as a specific and bioactive marker for Cordyceps.
CCP enables simultaneous qualitative and quantitative analysis, ensuring the authenticity and quality of Cordyceps.
This marker addresses the critical need for reliable identification in the functional food market.