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Replication-dependent Histone Biosynthesis Is Coupled To Cell-cycle Commitment.

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Replication-dependent Histone Biosynthesis Is Coupled To Cell-cycle Commitment.

Related Experiment Video

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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Published on: June 6, 2017

Replication-dependent histone biosynthesis is coupled to cell-cycle commitment.

Claire Armstrong^1,2, Sabrina L Spencer^3,2

¹Department of Biochemistry, University of Colorado, Boulder, CO 80303.

Proceedings of the National Academy of Sciences of the United States of America

|July 30, 2021

View abstract on PubMed

Summary

This summary is machine-generated.

Replication-dependent histone biosynthesis begins in G1, not just S phase. Cells build a small histone pool in G1 for DNA replication, with major production during S phase.

Keywords:

NPAT SLBP histone locus body replication-dependent histone restriction point

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

The established model links replication-dependent (RD) histone biosynthesis exclusively to DNA replication during S phase.
However, cell cycle regulators like E2F and CDK2 suggest earlier involvement in RD histone production.

Purpose of the Study:

To investigate the precise timing of RD histone biosynthesis initiation relative to the cell cycle.
To challenge the existing model and propose a revised understanding of histone production dynamics.

Main Methods:

Utilized fixed- and live-cell imaging techniques in human cells.
Observed and analyzed RD histone gene expression patterns throughout the cell cycle.

Main Results:

Demonstrated that RD histone biosynthesis initiates in the G1 phase of the cell cycle.

Revealed a hybrid model where histone production starts in G1 and significantly increases during S phase.

Identified a distinct molecular state between cells committed to cell division and those exiting the cell cycle.

Conclusions:

RD histone biosynthesis is initiated earlier than previously thought, starting in G1.
Cells committed to division establish an initial histone pool in G1 for upcoming DNA replication.
This finding refines our understanding of cell cycle control and histone regulation.