FREQUENCY OF PRESENCE OF PERIODONTOPATHOGENIC BACTERIA IN THE PERIODONTAL POCKETS

The study is aimd at detection the pathogenic markers: Aggregatibacter actinomycetemcomit, Porphyromonas Gingivalis, Prevotella Intermedia, Tannerella Forsythia and Treponema Denticola, identify their types, qualitative content and encounter frequency in periodontal pockets of the patients with generalized periodontitis, stage II (A or B level), before and after treatment with mechanical forms of therapy - Ultrasound, Vector or Laser Therapy . The material has been studied by using Micro-Ident biological method based on DNA-Strip technology. The surveyed 25 patients (women - 75% and men - 25%), 20 to 60 years of age, were divided into the three groups: group I treatment with Ultrasound scaler (n=9), group II treatment with Ultrasound Scaler and Diode laser (n=8), group III treatment with Ultrasound Scaler and Vector Sistem (n=8). All five strain before treatment were detected in 3 (12.5%) cases, none of the strain were detected in 1 patient (4%); among the rest 21 patients (87.5%) the following associations of different microorganisms were detected: P.G., P.I, T.F., T.D. - in 20.8% of patients, P.G., T.F., T.D. - 12.5%; A.A., P.G., P.I., T.F. - 4.16%; P.G., P.I., T.F.-33.28%; P.I., T.F., T.D. - 4.16%; A.A., P.G., T.F., T.D. - 12.5%; P.I., T.F. - 4.16%; P.G., P.I., T.D. - 4.16%. After evaluating the effectiveness of the treatment, based on Ultrasound system, Vector system, Diode laser and the complex picture of clinical and laboratory examinations in patients with generalized periodontitis, stage II, A or B level, a significant improvement in clinical conditions was observed. The microbiological study of Aggregatibacter Actinomycetemcomit (AA), Porphyromonas Gingivalis (PG), Prevotella Intermidia (PI), Tannerella Forsythia (BF), Treponema Denticola (TD) showed a complete elimination of qualitative and quantitative data after Vector therapy, but no reliable elimination of bacteria was observed after ultrasound and laser therapies. After treatment all five strain were detected in 1 (4%) case, 4 strain A.A., P.G., T.F., T.D. in 1 (4%) case, P.G.,P.I.,T.F.,T.D. - 2 (8%) case, 3 strain P.G., P.I., T.F in 3 (12.5%) case, 2 strain P.G., T.F - 1 strain (4%) and 1 T.F in 2 (8%).