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Performance Analysis of Non-Interferometry Based Surface Plasmon Resonance Microscopes.

Sorawit Tontarawongsa1, Sarinporn Visitsattapongse1, Suejit Pechprasarn2

  • 1Department of Biomedical Engineering, School of Engineering, King Mongkut's Institute of Technology, Ladkrabang, Bangkok 10520, Thailand.

Sensors (Basel, Switzerland)
|August 10, 2021
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Summary
This summary is machine-generated.

This study analyzes non-interferometric surface plasmon microscopes, finding tradeoffs between resolution and sensitivity. Annulus and half-annulus apertures offer optimal performance for biochemical sensing and biomolecular binding kinetics.

Keywords:
instrumentationquantitative measurementsurface plasmon microscopysurface plasmon resonance

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Area of Science:

  • Optics and Photonics
  • Biophysics
  • Materials Science

Background:

  • Surface plasmon microscopy is valuable for label-free, high-sensitivity detection in biochemical sensing and biomolecular binding kinetics.
  • Various non-interferometric surface plasmon microscope designs exist, each with unique performance characteristics.

Purpose of the Study:

  • To develop a theoretical framework for analyzing and comparing non-interferometric surface plasmon microscopes.
  • To identify strengths and weaknesses of different designs.
  • To quantify performance metrics like resolution, contrast, sensitivity, and accuracy.

Main Methods:

  • Theoretical analysis and comparison of six non-interferometric surface plasmon microscope configurations.
  • Evaluation of performance in both quantitative and non-quantitative imaging modes.
  • Analysis of factors influencing spatial resolution, including surface plasmon propagation length.

Main Results:

  • A tradeoff exists between image contrast and spatial resolution in non-quantitative imaging.
  • The half-annulus aperture yielded the highest sensitivity (127.058 rad/μm² RIU⁻¹), followed by the full annulus aperture (126.318 rad/μm² RIU⁻¹).
  • Annulus and half-annulus apertures demonstrated optimal resolution, sensitivity, and crosstalk; resolution depends on surface plasmon propagation length, not objective NA.

Conclusions:

  • Annulus and half-annulus aperture designs are recommended for optimal performance in surface plasmon resonance (SPR) microscopy.
  • For accurate SPR measurements, microfluidic channels and protein stamping should be at least 25 μm.
  • The study provides a framework for selecting and optimizing SPR microscopy techniques for various applications.