[A method of marking neurons on brain slices using patch clamp technique].

Area of Science:

• Neuroscience
• Cell Biology

Background:

• Patch clamp technique is crucial for electrophysiological studies.
• Visualizing recorded neurons post-experiment is essential for correlating function and morphology.

Purpose of the Study:

• To develop and validate a method for marking neurons using patch clamp.
• To enable morphological analysis of electrophysiologically characterized neurons.

Main Methods:

• Whole-cell patch clamp recording on vibratome-cut brain slices.
• Intracellular perfusion with Neurobiotin Tracer via microelectrode.
• Post-recording fixation, permeabilization, and Streptavidin-Texas Red staining.
• Fluorescence microscopy for visualization.

Main Results:

• Successful patch clamp recordings with stable voltage and current measurements.
• Demonstrated ability to induce action potentials in current clamp mode.
• Clear visualization of neuron morphology, including cell body and protrusions, post-staining.

Conclusions:

• The described method effectively marks neurons for morphological analysis after patch clamp.
• This technique is user-friendly, providing intuitive and clear imaging.
• It enhances the understanding of neuronal structure-function relationships.