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Multimodal Scanning Microscope Combining Optical Coherence Tomography, Raman Spectroscopy and Fluorescence Lifetime

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  • 1Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany.

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This study introduces a novel multimodal optical imaging system combining Optical Coherence Tomography (OCT), Fluorescence Lifetime Imaging Microscopy (FLIM), and Raman Spectroscopy (RS) for enhanced tissue diagnostics. This integrated approach enables detailed morpho-chemical characterization and correlation with H&E pathology slides.

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Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Clinical Pathology

Background:

  • Conventional bright-field microscopy lacks depth and molecular specificity.
  • Emerging optical modalities like RS, FLIM, and OCT offer rich molecular, chemical, and morphological data.
  • Integrating these modalities is crucial for maximizing their diagnostic potential.

Purpose of the Study:

  • To develop and implement a novel, compact, clinically applicable multimodal scanning microscope.
  • To enable co-registered acquisition of OCT, FLIM, and RS for mesoscale tissue investigation (0.1-5 mm).
  • To correlate multimodal spectroscopic data with Hematoxylin and Eosin (H&E) stained pathology slides for ground truth comparison.

Main Methods:

  • Development of a single device for simultaneous OCT, FLIM, and RS data acquisition.
  • Co-registered imaging allowing direct comparison of information from different modalities.
  • Preparation and characterization of H&E slides for correlation with optical imaging data.

Main Results:

  • Successful implementation of a multimodal imaging system combining OCT, FLIM, and RS.
  • Enabled correlated investigation of biological tissues at the mesoscale.
  • Demonstrated the ability to correlate multimodal spectroscopic signals with H&E stained pathology.

Conclusions:

  • The developed system is the first compact, clinically applicable multimodal microscope integrating OCT, FLIM, and RS.
  • It facilitates comprehensive morpho-chemical tissue characterization.
  • This technology allows direct correlation of optical data with pathological ground truth in a clinical setting.