Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Csf1r-mediated depletion of myeloid cells prevents dopaminergic neuron loss during chronic colitis.

Journal of neuroinflammation·2026

Same author

Spatial and multi-omic profiling reveals pericyte-derived CCL19 as a key prognostic factor in CNS lymphoma.

HemaSphere·2026

Same author

Bacterial infection reshapes monocyte and macrophage ontogeny at the CNS borders.

Science immunology·2026

Same author

Distinct and combined interferon-ɑ/β-receptor-1 loss in neurons and astrocytes disrupt brain energy metabolism and drive Parkinsonian dementia.

Journal of biomedical science·2026

Same author

High-dimensional Spatial Immune Profiling Highlights Microglia-Like Cells in Human Dorsal Root Ganglia.

European journal of immunology·2026

Same author

Sustaining microglial reparative function enhances stroke recovery.

Nature·2026

Same journal

Sparse component analysis: A method that uncovers separable computations within neural population activity.

Neuron·2026

Same journal

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens.

Neuron·2026

Same journal

TGF-β1-induced endothelial transcytosis drives blood-brain barrier leakage during aging.

Neuron·2026

Same journal

Image space opens up for visual neuroscience.

Neuron·2026

Same journal

Septal GLP-1 receptors control alcohol taking and seeking.

Neuron·2026

Same journal

Microglial fitness in moderation: Tuning TREM2 signaling through Ptpn6.

Neuron·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 24, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Current tools to interrogate microglial biology.

Anaelle Aurelie Dumas¹, Katharina Borst¹, Marco Prinz²

¹Institute of Neuropathology, Medical Faculty, University of Freiburg, Freiburg, Germany.

|August 14, 2021

Summary

This summary is machine-generated.

This guide explores cutting-edge methods for studying microglial cells in the central nervous system (CNS). It highlights advancements in single-cell technologies and mouse models for understanding microglial biology and disease.

More Related Videos

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

Related Experiment Videos

Last Updated: Oct 24, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

Area of Science:

Neuroscience
Immunology
Cell Biology

Background:

Microglial cells are crucial for central nervous system (CNS) development, homeostasis, and disease.
Technical advancements enable detailed analysis of microglial functions.
Single-cell technologies and mouse models have improved understanding of microglial heterogeneity.

Purpose of the Study:

To provide a guide to current cutting-edge methods for dissecting microglial biology.
To inform researchers about the advantages and limitations of available tools.
To ensure the correct application of novel techniques in microglial research.

Main Methods:

Single-cell technologies (e.g., single-cell RNA sequencing)
Targeted mouse models for genetic manipulation and cell tracking
Advanced imaging and functional assays

Main Results:

Deciphering microglial heterogeneity through advanced analytical systems.
Dissecting specific microglial functions in various physiological and pathological contexts.
Identifying limitations and advantages of current methodologies.

Conclusions:

A comprehensive understanding of microglial cell functions requires sophisticated analytical tools.
Awareness of method limitations is critical for accurate interpretation of results.
This guide facilitates the effective application of cutting-edge techniques in microglial research.