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Published on: September 8, 2013
Iridium-Triggered Allylcarbamate Uncaging in Living Cells
Neelu Singh1, Ajay Gupta1, Puja Prasad
1School of Physical Sciences, Jawaharlal Nehru University (JNU), New Delhi 110067, India.
Abstract:
Designing a metal catalyst that addresses the major issues of solubility, stability, toxicity, cell uptake, and reactivity within complex biological milieu for bioorthogonal controlled transformation reactions is a highly formidable challenge. Herein, we report an organoiridium complex that is nontoxic and capable of the uncaging of allyloxycarbonyl-protected amines under biologically relevant conditions and within living cells. The potential applications of this uncaging chemistry have been demonstrated by the generation of diagnostic and therapeutic agents upon the activation of profluorophore and prodrug in a controlled fashion within HeLa cells, providing a valuable tool for numerous potential biological and therapeutic applications.
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