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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
Published on: March 14, 2021
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Strategies for constructing pluripotent stem cell- and progenitor cell-derived three-dimensional cardiac
Xinyue Chu1, Mingyu Wang1,2, Xiaoyan Qiu3
1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing, China.
Journal of Biomedical Materials Research. Part A
|August 16, 2021
Summary
Three-dimensional (3D) cardiac micro-tissues offer a promising in vitro model for heart research. This review highlights strategies to overcome challenges in 3D cardiac micro-tissue development for improved heart disease therapies.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Cardiovascular Research
Background:
- Three-dimensional (3D) cardiac micro-tissues simulate heart structure and function in vitro, offering a platform for studying cardiac development, damage, and regeneration.
- This technology holds potential for developing novel therapies for heart diseases.
- Current limitations include incomplete microarchitecture, immune cell loss, poor reproducibility, and nutrient/waste exchange issues.
Purpose of the Study:
- To review scaffolds and cell sources for 3D cardiac micro-tissue construction.
- To explore strategies for maturing and functionalizing stem cell-derived cardiomyocytes.
- To identify major challenges and enable future fabrication of 3D cardiac micro-tissues or organoids.
Main Methods:
- Literature review of existing research on 3D cardiac micro-tissue fabrication.
- Analysis of strategies for cell sourcing and maturation.
- Identification of key challenges in current 3D cardiac micro-tissue technology.
Main Results:
- Scaffolds and cell resources are crucial for building functional 3D cardiac micro-tissues.
- Maturation strategies are essential for enhancing the function of stem cell-derived cardiomyocytes.
- Significant challenges remain in achieving complete microarchitecture, retaining immune cells, ensuring reproducibility, and optimizing nutrient supply.
Conclusions:
- Optimizing the construction of 3D cardiac micro-tissues and improving cell functions are critical.
- Addressing current limitations will enable advanced applications in drug screening, disease modeling, and regenerative treatments.
- Further research is needed to fully realize the potential of 3D cardiac micro-tissues and organoids.
