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Related Experiment Video

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Protocol to study starvation-induced autophagy in developing rat neurons.

José Wojnacki1, Sébastien Nola1, Thierry Galli1,2

  • 1Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, 75014 Paris, France.

STAR Protocols
|August 17, 2021
PubMed
Summary

This study introduces a simple method to induce autophagy in cultured neurons using nutrient deprivation. This protocol aids in studying the complex role of autophagy in neuronal function.

Keywords:
AntibodyCell BiologyCell cultureMicroscopyNeuroscience

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Autophagy plays a crucial role in various cellular pathways.
  • The effects of modulating autophagy in neurons are complex and not fully understood.

Purpose of the Study:

  • To present a straightforward protocol for inducing autophagy in primary neurons.
  • To facilitate the investigation of autophagy's role in neuronal cells.

Main Methods:

  • Induction of autophagy via partial nutrient deprivation in primary neurons.
  • Utilizing neurons with or without transfection of plasmids encoding the Longin domain of VAMP7 or a nanobody against VAMP7.

Main Results:

  • Successful induction of autophagy in cultured neurons using the described protocol.
  • The protocol is effective in both transfected and non-transfected neuronal cells.

Conclusions:

  • The developed protocol offers a simple yet effective method to study autophagy in neuronal cultures.
  • This technique can advance research into the complex functions of autophagy in neurons.