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Updated: Oct 23, 2025
Protocol for Long Duration Whole Body Hyperthermia in Mice
Published on: August 25, 2012
Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and
ChangDong Lin1, ZhaoYuan Liu1, Yue Li1
1State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
Abstract:
Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to Lin et al. (2019).
