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Updated: Oct 23, 2025

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
Ustekinumab therapy changes the transcriptional activity pattern of TGF-β1-3 genes
Beniamin Grabarek1,2,3, Dominika Wcisło-Dziadecka4, Barbara Strzałka-Mrozik3
1Katowice School of Technology, University of Science and Art, Katowice, Poland.
Introduction:
One of the examples of genes whose expression can be altered by the action of ustekinumab is TGF-β. It is a pleiotropic cytokine whose activity affects psoriatic changes and the state of homeostasis of the whole organism.
Aim:
To evaluate the effect of ustekinumab on the transcriptional activity of TGF-β family genes in patients with psoriatic arthritis and to check whether the results obtained can be helpful in monitoring the progress of treatment.
Material And Methods:
From total PBMCs obtained from peripheral blood of 14 patients with psoriatic arthritis, total RNA was isolated. The expression level of the TGF-β1, TGF-β2 and TGF-β3 genes was determined by RT-qPCR in real time.
Results:
In all the analysed samples, the presence of mRNA of three TGF-β isoforms was quantitated in each week of therapy. TGF-β3 and the smallest TGF-β2 showed the highest expression. Statistically significant correlations were observed in the amount of TGF-β1 and TGF-β3/µg mRNA RNA, TGF-β2 and TGF-β2/µg RNA and TGF-β3 and TGF-β3/µg RNA.
Conclusions:
Ustekinumab influences the transcriptional activity of TGF-β genes, and the changes caused have a bearing on the patient's health.
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