Related Experiment Video
Updated: Oct 23, 2025
Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
Published on: November 10, 2023
Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH
Sascha Tierling1, Kathrin Kattler1, Markus Vogelgesang2
1Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
Background:
The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations.
Methods:
Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions.
Results:
The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours.
Conclusions:
We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.
