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Establishment of an Experimental System for Intraperitoneal Chemotherapy in a Rat Model
Sunwoo Park1, Soo Jin Park2, Hee Su Lee3
1Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Aim:
To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model.
Materials And Methods:
We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods.
Results:
The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC.
Conclusion:
This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.
