Optimization of the phototrophic Cyanobacteria polyhydroxybutyrate (PHB) production by kinetic model simulation

Cyanobacteria can grow using inorganic substrates, such as CO₂ from industrial sources and nutrients from wastewaters, and therefore are promising microorganisms to produce polyhydroxybutyrate in a cleaner circular context. However, this biotechnological production is highly challenging because it involves different interlinked reactions that are affected by environmental conditions, which hinders process optimization. In this study a new biokinetic mechanistic model using novel experimental approaches was developed to optimize polyhydroxybutyrate (PHB) and glycogen production. The model includes, for the first time, the production of glycogen and its conversion into PHB, which has been found as the main pathway to produce PHB. Model was successfully (r²: 0.6-0.99) calibrated and validated with experimental data from photobioreactors inoculated with Synechocystis sp. The developed model was used to determine suitable initial conditions for a lab scale batch reactor (6.4 mgN·L^-1 and 2 mgP·L^-1) and a new configuration for the continuous industrial production of PHB was proposed and optimized using this tool. The maximum productivity (5.1 mgPHB·L^-1·d^-1) and the optimal configuration and operation of the serial reactors to produce PHB in an industrial scale was achieved using a hydraulic retention time of 4 days in the growth reactor. Then, this reactor daily fed 20 batch accumulation reactors, which were discharged after 20 days. The optimal influent nutrients concentrations for this configuration was found to be 50 mgN·L^-1 and 10 mgP·L^-1. Results found in this study show the necessity to optimize biopolymers production with Cyanobacteria considering environmental conditions, and demonstrated the potential of this model as a tool to increase PHB productivity.