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From sieve to microscope: An efficient technique for sample transfer in the process of microplastics' quantification.
Julia A Prume1,2, Felix Gorka3, Martin G J Löder4
1Department of Physics, Philipps University Marburg, Germany.
Methodsx
|August 25, 2021
Summary
A new four-step method improves microplastic quantification by reducing particle loss during sample preparation. This technique enhances the accuracy and reproducibility of microplastic analysis in environmental samples.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Materials Science
Background:
- Accurate quantification of microplastics is essential for environmental monitoring.
- Current methodologies often lack reproducibility and detailed reporting of sample preparation steps.
- Particle loss during sample fractionation and transfer is a significant source of error.
Purpose of the Study:
- To present a reliable and reproducible four-step technique for microplastic sample preparation and microscopic examination.
- To address the need for improved methodology in microplastic quantification.
- To minimize particle loss during sample processing for environmental samples.
Main Methods:
- Size-fractionation using a sieve cascade.
- Vacuum filtration onto a filter after rinsing from sieves.
- Transferring particles to a petri dish with minimal water for microscopic examination.
- Microscopic analysis under transmitted light in wet or dry conditions.
Main Results:
- The technique is suitable for various environmental samples like water, sediment, and soil.
- It is effective for microplastic fragments larger than 300 µm.
- Validation with polypropylene showed an average recovery of 94% ± 13.5% (mean) and 100% (median).
Conclusions:
- The presented four-step method offers a reliable approach for microplastic sample preparation.
- It enhances sample transfer and concentration, leading to more accurate quantification.
- The technique utilizes standard laboratory equipment and is applicable to diverse environmental matrices.
