A label-free ratiometric method to detect Hg²⁺ based on structural change of DNA

In this work, a simple ratiometric method has been designed to detect Hg²⁺ based on the structural change between double-stranded DNA (dsDNA) and its G-quadruplex structure. When Hg²⁺ was added, the designed G-quadruplex structure could change into the corresponding dsDNA by forming the T-Hg²⁺ -T mismatch. This kind of variation resulted in a decrease in the fluorescence of the G-quadruplex/N-methyl mesoporphyrin IX (NMM) complex and an increase in the fluorescence from the dsDNA/SYBR Green I (SG I) pair. The secondary excitation wavelength of SG I was used to excite NMM and SG I simultaneously. The titration experiment indicated that the new method had a linear response within 0.7-2.5 μM Hg²⁺ with a limit of detection of 9.3 nM. Because using the T-Hg²⁺ -T mismatch to recognize Hg²⁺ was very specific, the selectivity of the new method was also satisfactory. The recoveries ranged from 92.8% to 110.2% suggested that this new method could achieve a potential application for Hg²⁺ detection in real environmental samples.