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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Oct 22, 2025

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Quantification and mapping of DNA modifications.

Yi Dai¹, Bi-Feng Yuan^1,2, Yu-Qi Feng^1,2

¹Sauvage Center for Molecular Sciences, Department of Chemistry, Wuhan University Wuhan 430072 P. R. China bfyuan@whu.edu.cn +86-27-68755595 +86-27-68755595.

RSC Chemical Biology

|August 30, 2021

Summary

This summary is machine-generated.

DNA modifications regulate biological processes and are linked to diseases. Recent analytical advancements enable sensitive detection, quantification, and genome-wide mapping of these crucial DNA changes.

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Last Updated: Oct 22, 2025

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DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

DNA molecules contain natural modifications beyond canonical nucleobases.
These modifications play critical roles in regulating biological processes, cell differentiation, and development.
Dysregulation of DNA modifications is implicated in various human diseases.

Purpose of the Study:

To provide an overview of recent advances in analytical methods and techniques for natural DNA modifications.
To discuss the principles, advantages, and limitations of developed methods for quantification and genome-wide mapping.
To highlight the importance of these methods in deciphering the biological roles of DNA modifications.

Main Methods:

Review of recent analytical methods and techniques for DNA modification detection.
Discussion of approaches for sensitive detection, accurate quantification, and genome-wide mapping.
Analysis of principles, advantages, and limitations of current methodologies.

Main Results:

Numerous new DNA modifications have been discovered in prokaryotic and eukaryotic genomes.
Advanced analytical techniques facilitate sensitive detection and accurate quantification.
Genome-wide mapping of DNA modifications is increasingly feasible.

Conclusions:

Innovations in analytical methods are significantly advancing the study of DNA modifications.
Sensitive detection, accurate quantification, and genome-wide mapping are essential for understanding DNA modification functions.
Future methodological developments are expected to overcome current challenges and accelerate research in this field.