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Polymerase κ Recruits DDX23 To Promote R-Loop Resolution.

Feng Tang1,2, Yinan Wang1, Jun Yuan1

  • 1Department of Chemistry, University of California, Riverside, California 92521-0403, United States.

Analytical Chemistry
|April 8, 2026
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Summary
This summary is machine-generated.

Polymerase kappa (Pol κ) interacts with DDX23 to resolve R-loops, structures that threaten genome stability. This collaboration helps maintain genomic integrity by clearing these DNA structures.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Genome stability is crucial and threatened by DNA damage and R-loops.
  • Translesion synthesis (TLS) pathways, including polymerase kappa (Pol κ), are vital for managing DNA lesions.
  • Pol κ is known to bypass certain DNA adducts and participate in DNA repair.

Purpose of the Study:

  • To identify Pol κ interacting proteins and elucidate its function in maintaining genome stability.
  • To investigate the role of Pol κ in the resolution of R-loops.

Main Methods:

  • Proximity labeling and affinity pull-down assays were used to identify Pol κ interactors.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed for proteomic analysis.
  • Cellular localization and R-loop accumulation were assessed upon Pol κ and DDX23 manipulation.

Main Results:

  • Pol κ was found to interact with DDX23.
  • Pol κ localizes to R-loop sites within the chromatin.
  • Pol κ recruits DDX23 to R-loop sites, facilitating R-loop resolution.
  • Ablation of either Pol κ or DDX23 leads to increased R-loop accumulation.

Conclusions:

  • A novel interaction between Pol κ and DDX23 was discovered.
  • Pol κ and DDX23 collaborate to resolve R-loops, thereby maintaining genome stability.
  • This Pol κ-DDX23 pathway is essential for preventing R-loop accumulation.