Next-Generation Sequencing Analysis of 8-Oxo-7,8-dihydroguanine in Human Mitochondrial DNA

Oxidative damage to mitochondrial DNA is closely associated with the development of various human diseases. 8-Oxo-7,8-dihydroguanine (8-oxoG) is a vital biomarker of oxidative DNA damage with a putative role as an epigenetic-like modification. Herein, we reported a high-throughput sequencing analysis of 8-oxoG modifications using a chemical labeling-based method and identified multiple 8-oxoG sites in human mitochondrial DNA. We also demonstrated that Y-box binding protein 1 (YBX1), an 8-oxoG-binding protein, is potentially involved in the transcriptional regulation of mitochondrial genes containing 8-oxoG modification sites. These findings provide a solid foundation for further understanding the biological consequences of 8-oxoG modification in mammalian cells.