Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chemotaxis and Direction of Cell Migration

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineered intestinal crypt geometry uncovers YAP1-dependent fetal-to-adult transition.

Cell stem cell·2026

Same author

Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation.

PLoS computational biology·2026

Same author

Chemical and Mechanical Regulation of Leukocyte Migration.

Cold Spring Harbor perspectives in biology·2025

Same author

Visualising Neutrophil Actin Dynamics in Zebrafish in Response to Laser Wounding Using Two-Photon Microscopy.

Bio-protocol·2024

Same author

Management of inguinal lymph nodes in locally advanced, surgically unresectable, squamous cell carcinoma of the vulva.

Gynecologic oncology·2024

Same author

A two-step search and run response to gradients shapes leukocyte navigation in vivo.

The Journal of cell biology·2022

Same journal

Transcriptomic signatures and neural cell composition in schizophrenia iPSC-derived neuron cultures.

Cells & development·2026

Same journal

Upregulation of adenylyl cyclase 1 is crucial for osteogenic and adipogenic differentiation of MSCs.

Cells & development·2026

Same journal

Toll-like receptors in epithelial mechanics and surveillance.

Cells & development·2026

Same journal

Functions and mechanisms of BRCA1 in early embryonic development.

Cells & development·2026

Same journal

The face you're born with: How early development dictates a lifetime of expression and ageing.

Cells & development·2026

Same journal

Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.

Cells & development·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 22, 2025

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

Neutrophil motion in numbers: How to analyse complex migration patterns.

Antonios Georgantzoglou¹, Joanna Matthews¹, Milka Sarris¹

¹Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Site, Cambridge CB2 3DY, UK.

Cells & Development

|August 30, 2021

Summary

This summary is machine-generated.

Live imaging reveals neutrophils exhibit novel swarming and reverse migration behaviors. This study provides quantitative analysis methods for these immune cell dynamics in inflammation.

Keywords:

Clustering Neutrophil swarming Quantitative analysis Reverse migration

More Related Videos

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Published on: January 7, 2015

Related Experiment Videos

Last Updated: Oct 22, 2025

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Published on: January 7, 2015

Area of Science:

Immunology
Cell Biology
Biophysics

Background:

In vivo imaging transformed the study of leukocyte trafficking and immune cell dynamics.
Neutrophil migration, crucial in inflammation, was previously thought to be solely extrinsically driven and resolved by apoptosis.
Live imaging revealed neutrophils exhibit self-organized migration ('swarming') and exit from inflammation sites ('reverse migration').

Purpose of the Study:

To provide recommendations for the quantitative analysis of neutrophil swarming and reverse migration.
To introduce relevant analysis tools to researchers in the field.
To establish common frameworks and standards for studying these neutrophil behaviors.

Main Methods:

Utilizing in vivo imaging techniques in zebrafish and mice models.
Observing and documenting neutrophil migration patterns in inflammatory environments.
Developing and summarizing statistical analysis approaches for quantifying cell behaviors.

Main Results:

Neutrophils demonstrate self-organization in migration through collective gradient generation (swarming).
Neutrophils can actively leave inflammatory sites (reverse migration).
Quantitative analysis is essential for understanding these complex neutrophil dynamics.

Conclusions:

Neutrophil migration is more complex than previously understood, involving collective behaviors.
Standardized quantitative analysis is crucial for advancing the study of neutrophil swarming and reverse migration.
This work aims to facilitate consistent and robust research in neutrophil dynamics.