Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineered intestinal crypt geometry uncovers YAP1-dependent fetal-to-adult transition.

Cell stem cell·2026

Same author

Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation.

PLoS computational biology·2026

Same author

Chemical and Mechanical Regulation of Leukocyte Migration.

Cold Spring Harbor perspectives in biology·2025

Same author

A two-step search and run response to gradients shapes leukocyte navigation in vivo.

The Journal of cell biology·2022

Same author

Chondrocyte-specific response to stiffness-mediated primary cilia formation and centriole positioning.

American journal of physiology. Cell physiology·2022

Same author

Neutrophil motion in numbers: How to analyse complex migration patterns.

Cells & development·2021

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 24, 2025

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Published on: December 4, 2020

Visualising Neutrophil Actin Dynamics in Zebrafish in Response to Laser Wounding Using Two-Photon Microscopy.

Ivanna Williantarra¹, Antonios Georgantzoglou¹, Milka Sarris¹

¹Department of Physiology Development and Neuroscience, University of Cambridge, Cambridge, UK.

|June 14, 2024

Summary

This study introduces a novel laser-wound assay in zebrafish larvae to observe cell migration and cytoskeletal dynamics in response to chemical gradients during tissue injury and immune responses.

Keywords:

Actin dynamics Cell migration Chemotaxis Laser wounding Neutrophil Two-photon imaging

More Related Videos

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Published on: January 30, 2013

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Published on: February 3, 2017

Related Experiment Videos

Last Updated: Jun 24, 2025

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Published on: December 4, 2020

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Published on: January 30, 2013

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Published on: February 3, 2017

Area of Science:

Cell Biology
Developmental Biology
Immunology

Background:

Cell migration, or chemotaxis, is crucial for development, wound healing, and immune responses.
Neutrophils are key migratory cells recruited to inflammatory sites, with their chemotaxis dependent on cytoskeletal dynamics.
Existing in vitro assays limit the study of neutrophil chemotaxis in complex living tissues.

Purpose of the Study:

To develop and describe a new laser-wound assay for monitoring cellular behavior and cytoskeletal dynamics in response to chemical gradients in living zebrafish larvae.
To enable high-resolution visualization of cellular responses immediately following acute injury.
To provide a versatile platform for further studies, including genetic or chemical perturbations.

Main Methods:

Generation of focal injury in zebrafish larvae using a two-photon microscope laser-wound assay.
Utilizing fluorescent reporters (e.g., Lifeact-mRuby) to visualize dynamic actin and cellular behavior.
Live imaging, cell segmentation, and quantification of actin dynamics post-wounding.

Main Results:

The assay allows real-time monitoring of cellular behavior and actin dynamics immediately after exposure to chemical gradients generated by laser-induced injury.
Two-photon intravital microscopy ensures precise, deep tissue injury, superior to one-photon methods.
The protocol is effective for studying neutrophil swarming and can be adapted for infectious disease models.

Conclusions:

The developed laser-wound assay provides a powerful tool for studying cellular chemotaxis and cytoskeletal dynamics in vivo with high resolution.
This method facilitates the investigation of cellular responses to acute injury in complex biological systems.
The assay's adaptability makes it valuable for diverse research applications in cell migration and immunology.