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Accurate and efficient intracellular delivery biosensing system by nanostrawed electroporation array.
Jiaru Fang1, Jiarong Xu2, Yuting Xiang2
1State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
A novel intracellular delivery biosensing system using nanostrawed electroporation array (NEA) offers a faster and more accurate method for assessing electroporation performance, improving cell viability and delivery efficiency in biomedical research.
Area of Science:
- Biotechnology
- Cell Biology
- Medical Technology
Background:
- Electroporation is a key technique for introducing molecules into cells by transiently increasing membrane permeability.
- Current methods for assessing electroporation efficiency, such as microscopy and flow cytometry, are often inaccurate, labor-intensive, and time-consuming.
- There is a need for more precise and efficient methods to evaluate electroporation performance in situ.
Purpose of the Study:
- To develop an intracellular delivery biosensing system for efficient and accurate assessment of universal electroporation performance.
- To evaluate cell viability, delivery efficiency, and cell mortality using the developed system.
- To establish a promising universal platform for assessing nanodevice electroporation.
Main Methods:
- Development of a nanostrawed electroporation array (NEA) integrated with a fluorescent microscope and automated analysis software.
- Implementation of an enhanced fluorescent watershed segmentation (enhanced FWS) algorithm for automated analysis.
- Comparison of the developed system's accuracy and speed against conventional methods.
Main Results:
- The developed intracellular delivery biosensing system accurately assesses electroporation performance, including cell viability, delivery efficiency, and cell mortality.
- The enhanced FWS algorithm demonstrated low deviation (~5%) and significantly reduced analysis time (~8 seconds per 10 images).
- Conventional methods showed higher deviation (~13%) and considerably longer analysis times (~10 minutes per 10 images).
Conclusions:
- The intracellular delivery biosensing system provides a highly accurate and efficient method for evaluating electroporation.
- This system significantly improves upon the limitations of conventional, labor-intensive analytical strategies.
- The developed system is expected to be a valuable universal platform for nanodevice electroporation assessment in various scientific fields.
