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Updated: Oct 21, 2025

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
Published on: September 18, 2019
19 F-detected dual-optimized inverted 1 JCC 1,n-ADEQUATE
Ronald C Crouch1, Jeffrey G Raab2, Marius Pelmuş3,4
1Analytical Instruments, JEOL USA Inc., Peabody, MA, USA.
Abstract:
Modification of the recently reported 19 F-detected 1,1-ADEQUATE experiment that incorporates dual-optimization to selectively invert a wide range of 1 JCC correlations in a 1,n-ADEQUATE experiment is reported. Parameters for the dual-optimization segment of the pulse sequence were modified to accommodate the increased size of 1 JCC homonuclear coupling constants of poly- and perfluorinated molecules relative to protonated molecules to allow broadband inversion of the 1 JCC correlations. The observation and utility of isotope shifts are reported for the first time for 1,1- and 1,n-ADEQUATE correlations.
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