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Three-Dimensional Printing to Build Fibrous Protein Architectures
1Biomass Molecular Engineering Center and Department of Materials Science and Engineering, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, Anhui, China.
Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2021
Summary
This study details protocols for creating fibrous protein bioinks for 3D printing. These advanced materials, including silk fibroin and collagen, enable sophisticated biomedical and materials science applications.
Area of Science:
- Biomaterials Science
- Biomedical Engineering
- Materials Science
Background:
- Fibrous proteins are key bioinks for 3D printing complex structures.
- Optimizing cross-linking and rheology is crucial for manufacturing protein-based inks.
- 3D printing techniques like extrusion, droplet, and light-assisted methods are used for fibrous protein architectures.
Purpose of the Study:
- To present protocols for fabricating various fibrous protein inks.
- To demonstrate the use of these bioinks in 3D printing sophisticated structures.
- To cover bioinks derived from silk fibroin, collagen, fibrin, gelatin, and recombinant spider silk.
Main Methods:
- Development of protocols for fibrous protein ink formulation.
- Application of extrusion-based, droplet-based, and light-assisted 3D printing techniques.
- Characterization of ink properties for printability.
Main Results:
- Successful fabrication of diverse fibrous protein bioinks.
- Demonstrated ability to 3D print intricate structures using these bioinks.
- Established protocols applicable to various printing technologies.
Conclusions:
- Fibrous protein bioinks offer versatile platforms for advanced 3D printing.
- Tailoring ink properties is essential for successful fabrication of biomedical and materials science applications.
- The presented protocols facilitate the creation of sophisticated 3D printed constructs.
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