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Related Experiment Video

Updated: Oct 21, 2025

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Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing.

Markus Seeger1,2, Christoph Dehner1,2, Dominik Jüstel1,2,3

  • 1Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.

Communications Biology
|September 7, 2021
PubMed
Summary
This summary is machine-generated.

Concurrent 5-modal microscopy (Co5M) enables label-free, in vivo visualization of biological processes. This advanced technique captures multiple contrasts simultaneously, offering unprecedented insights into complex physiological and pathological changes without disturbing the biological system.

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Area of Science:

  • * Biomedical imaging
  • * In vivo microscopy
  • * Label-free imaging

Background:

  • * Non-invasive investigation of biological processes is challenging due to the need for multiple contrast mechanisms.
  • * Intravital microscopy typically requires transgenic labels, which can disturb biological systems.
  • * Existing methods are limited in simultaneously capturing diverse contrast mechanisms for comprehensive analysis.

Purpose of the Study:

  • * To introduce concurrent 5-modal microscopy (Co5M) for label-free in vivo observation.
  • * To demonstrate Co5M's capability in simultaneously capturing optoacoustic, two-photon excitation fluorescence, second and third harmonic generation, and brightfield contrasts.
  • * To apply Co5M for non-invasive visualization and quantitative monitoring of wound healing biomarkers.

Main Methods:

  • * Development and application of concurrent 5-modal microscopy (Co5M).
  • * Simultaneous capture of five contrast mechanisms: optoacoustic, two-photon excitation fluorescence, second harmonic generation, third harmonic generation, and brightfield.
  • * Label-free in vivo imaging applied to wound healing models.

Main Results:

  • * Co5M successfully visualized multiple wound healing biomarkers non-invasively.
  • * Quantitative monitoring of wound shape, microvascular and collagen density, vessel size and fractality, and sebaceous gland plasticity was achieved.
  • * Detailed insights into the interplay of wound closure, vasodilation, angiogenesis, skin contracture, and epithelial reformation were obtained.
  • * Analysis revealed dynamic spatial and temporal changes inaccessible by other methods.

Conclusions:

  • * Co5M provides a novel platform for label-free, multi-contrast in vivo imaging.
  • * The technique offers unique insights into complex biological processes, such as wound healing.
  • * Co5M challenges conventional biological observation by enabling simultaneous acquisition of multiple pathophysiological parameters without labels.