In vitro in vivo studies on removal of middle molecules by macroporous resins

Various types of macroporous resin beads were screened for the adsorption of middle molecules in vitro. In order to obtain high adsorption capacity, the pore size of the resin should be large enough to allow diffusion of the middle molecules. The adsorption capacity of X-5 resin for Vitamin B12, inulin and cytochrome C was 35.3 mg/g, 1.2 mg/g and 42.9 mg/g respectively in vitro tests. Hemoperfusion with acute renal failure rat model showed good clearance (0.35 +/- 0.25) with X-5 resin for middle molecules.