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High sensitivity glycomics in biomedicine
Guinevere S M Lageveen-Kammeijer1, Bernhard Kuster2, Dietmar Reusch3
1Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
Mass Spectrometry Reviews
|September 8, 2021
Summary
This review highlights sensitive mass spectrometry methods for analyzing complex glycans and glycoproteins, crucial for biomedical research with limited sample sizes. Innovations in glycomic workflows enhance detection of N-linked and O-linked glycans.
Area of Science:
- Biomedical Mass Spectrometry
- Glycomics and Glycoproteomics
Background:
- Biomedical samples containing glycans and glycoproteins are often highly heterogeneous, complex, and available in minute quantities.
- These characteristics present significant analytical challenges, necessitating highly sensitive detection methods and workflows.
Purpose of the Study:
- To evaluate mass spectrometric workflows and detection methods for the analysis of glycans and glycoproteins.
- To highlight innovations in glycomic methodologies for high-sensitivity analysis of mammalian N-linked and GalNAc-type O-linked glycans.
Main Methods:
- Review of existing and emerging mass spectrometry-based analytical strategies.
- Focus on methodologies encompassing enzymatic treatments, chemical derivatization, purification, separation, and detection.
- Evaluation of techniques tailored for high sensitivity and complex biological matrices.
Main Results:
- Identification of key mass spectrometric workflows suitable for sensitive glycan and glycoprotein analysis.
- Highlighting of innovative glycomic techniques that improve the efficiency and sensitivity of sample preparation and analysis.
- Specific focus on advancements relevant to N-linked and O-linked glycan structures in mammals.
Conclusions:
- Sensitive mass spectrometry workflows are essential for overcoming analytical challenges in glycomics and glycoproteomics.
- Recent innovations in glycomic methodologies significantly enhance the capability to analyze complex glycan structures from limited biological samples.
- The reviewed approaches provide a foundation for advancing research in glycobiology and its biomedical applications.
