Real-time 3D single-molecule localization microscopy analysis using lookup tables
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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Published on: June 30, 2018
Fabian Hauser1,2, Jaroslaw Jacak1,2
1University of Applied Sciences, Upper Austria School of Medical Engineering and Applied Social Sciences, Garnisonstraße 21, 4020 Linz, Austria.
Abstract:
Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant.
