Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

7.8K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
7.8K
Structure and Function of Platelets01:18

Structure and Function of Platelets

2.2K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep Learning Models for Detection of Periapical Radiolucent Lesions on Panoramic Radiographs: A Systematic Review and Meta-Analysis.

International endodontic journal·2026
Same author

Genetic regulation of platelet-derived BDNF by rs11030119: discrepancy between circulating levels and hemostatic function.

Genes and immunity·2026
Same author

The dual role of the brain-derived neurotrophic factor as a regulator of hemostasis and thrombotic risk.

Current opinion in hematology·2026
Same author

Up to Date Status and Acceptance of the Influenza Vaccine among a National Sample of Emergency Department Patients.

The Journal of emergency medicine·2026
Same author

Tracking of Transplanted Stem Cells in Mouse Models Using Practical Fluorescence-Based Protocols.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Radiation-Induced Salivary Gland Fibrosis: Mechanisms, Emerging Therapies, and Gelatin-Based Bioengineered Models.

Gels (Basel, Switzerland)·2026
Same journal

Large Language Models and Retrieval-Augmented Platforms for the Diagnosis and Management of Periodontal Diseases: A Blinded Expert-Rated Comparative Study of 11 Systems.

Journal of clinical periodontology·2026
Same journal

Local Sodium Tanshinone IIA Sulfonate Attenuates Periodontal Bone Loss by Modulating Inflammatory, Immune and Osteoclastogenic Signalling.

Journal of clinical periodontology·2026
Same journal

Correction to ''Clinical Efficacy of Interventions Based on Professional Mechanical Plaque Removal in the Treatment of Dental Biofilm-Induced Gingivitis: A Systematic Review and Meta-Analysis''.

Journal of clinical periodontology·2026
Same journal

Extracellular Vesicles From Young Brain-Mediated Delivery of PRDX3 Ameliorates Alveolar Bone Loss in Periodontitis.

Journal of clinical periodontology·2026
Same journal

Cost Consequence Analysis of Non-Surgical Treatment Approaches for Patients With Periodontitis.

Journal of clinical periodontology·2026
Same journal

Management of Non-Plaque-Induced Gingival Conditions: A Systematic Review-Part 2: Inflammatory and Immune Conditions; Neoplasms; and Gingival Pigmentation.

Journal of clinical periodontology·2026
See all related articles

Related Experiment Video

Updated: Oct 20, 2025

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
07:51

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

Published on: March 19, 2018

9.3K

Differences in platelet-rich plasma composition influence bone healing.

Faez Saleh Al-Hamed1, Lina Abu-Nada1, Rania Rodan1

  • 1Faculty of Dentistry, McGill University, Montreal, Canada.

Journal of Clinical Periodontology
|September 13, 2021
PubMed
Summary
This summary is machine-generated.

The preparation method for leucocyte- and platelet-rich plasma (L-PRP) significantly impacts its composition and effectiveness in enhancing bone regeneration. Double-spin L-PRP demonstrated superior bone healing in vivo compared to other methods.

Keywords:
bone formationbone healingcritical size defectplatelet concentratesplatelet-rich plasmarat surgery

More Related Videos

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

18.7K
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
08:14

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

Published on: September 29, 2015

13.1K

Related Experiment Videos

Last Updated: Oct 20, 2025

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
07:51

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

Published on: March 19, 2018

9.3K
Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

18.7K
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
08:14

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

Published on: September 29, 2015

13.1K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Surgery

Background:

  • Platelet-rich plasma (PRP) is an autologous biomaterial used to promote bone regeneration.
  • Clinical outcomes with PRP for bone healing have been inconsistent.
  • Variations in PRP composition, particularly leucocyte content, may explain these discrepancies.

Purpose of the Study:

  • To investigate how different preparation methods influence leucocyte- and platelet-rich plasma (L-PRP) composition.
  • To assess the impact of these compositional changes on bone defect healing in an in vivo model.

Main Methods:

  • L-PRPs were prepared using single-spin, double-spin, and filtration centrifugation techniques.
  • Bone regeneration was evaluated in critical-size rat tibial defects treated with different L-PRP preparations or controls.
  • Micro-computed tomography (micro-CT) and histological analyses were performed after 2 weeks.

Main Results:

  • Double-spin L-PRP exhibited higher platelet concentrations than single-spin or filtered L-PRP.
  • Filtered L-PRP showed reduced mineral and metabolite concentrations.
  • Defects treated with double-spin L-PRP showed significantly better bone healing, with smaller defect volumes and reduced inflammatory cell infiltration compared to other L-PRP groups.

Conclusions:

  • The method used to prepare L-PRP critically affects its composition.
  • Optimizing L-PRP preparation, such as employing a double-spin method, can enhance its bone regenerative potential.
  • These findings highlight the importance of standardized L-PRP preparation for consistent clinical outcomes in bone regeneration.