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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Updated: Oct 20, 2025

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
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Next-generation sequencing and clinical histocompatibility testing.

Caleb Cornaby1, John L Schmitz2, Eric T Weimer2

  • 1McLendon Clinical Laboratories, UNC Health, Chapel Hill, NC, USA.

Human Immunology
|September 15, 2021
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) enhances histocompatibility testing for organ and stem cell transplants. This technology improves donor identification, risk assessment, and monitoring of transplant patients, with broad future applications.

Keywords:
HLAHistocompatibilityNGSNext-generation sequencingTransplant

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Area of Science:

  • Immunogenetics
  • Transplant Science
  • Genomic Medicine

Background:

  • Histocompatibility testing is crucial for successful transplantation, guiding donor selection and assessing risks.
  • Monitoring for donor-specific antibodies post-transplant is vital for patient outcomes.
  • Human Leukocyte Antigen (HLA) typing is a cornerstone of histocompatibility assessment.

Purpose of the Study:

  • To review the impact of Next-Generation Sequencing (NGS) on clinical histocompatibility testing.
  • To explore the current and future applications of NGS in transplantation and non-transplant settings.
  • To highlight advancements in Human Leukocyte Antigen (HLA) typing using NGS.

Main Methods:

  • Review of current literature on NGS-based HLA typing.
  • Analysis of NGS applications in solid organ and hematopoietic stem cell transplantation.
  • Discussion of NGS utility in HLA disease association studies.

Main Results:

  • NGS has significantly improved the accuracy and resolution of HLA typing.
  • NGS facilitates better donor identification and risk stratification in transplant candidates.
  • NGS aids in identifying specific alleles for monitoring post-transplant complications.

Conclusions:

  • NGS is revolutionizing histocompatibility testing, offering enhanced capabilities for transplant medicine.
  • The adoption of NGS technologies promises further advancements in clinical diagnostics and research.
  • NGS-based HLA typing is becoming indispensable for both transplant and non-transplant applications.