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Temperature-responsive spin column for sample preparation using an all-aqueous eluent.

Kenichi Nagase1, Yuta Ishizawa1, Masakazu Inoue1

  • 1Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo, 105-8512, Japan.

Analytica Chimica Acta
|September 18, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel temperature-responsive spin column for simple serum sample preparation. The column effectively removes proteins, enabling accurate serum drug analysis by simply adjusting temperature.

Keywords:
BioseparationSample preparationTemperature-responsive chromatographyTemperature-responsive polymer

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biochemistry

Background:

  • Serum drug analysis requires efficient protein removal for accurate quantification.
  • Existing methods for serum sample preparation can be complex and time-consuming.

Purpose of the Study:

  • To develop a simple, temperature-responsive spin column for serum protein removal.
  • To enable efficient sample preparation for serum drug analysis using an all-aqueous eluent.

Main Methods:

  • Grafting silica beads with temperature-responsive copolymers: poly(N-isopropylacrylamide-co-N,N-dimethylaminopropyl acrylamide-co-n-butyl methacrylate) (P(NIPAAm-co-DMAPAAm-co-BMA)) and poly(N-isopropylacrylamide-co-n-butyl methacrylate) (P(NIPAAm-co-BMA)).
  • Packing the beads into a double-layer spin column.
  • Evaluating purification efficacy using a model analyte (voriconazole) and serum proteins at varying temperatures (40°C and 4°C).

Main Results:

  • The spin column effectively adsorbed serum proteins and voriconazole at 40°C through hydrophobic and electrostatic interactions.
  • Decreasing the temperature to 4°C selectively eluted voriconazole with pure water, while serum proteins remained adsorbed.
  • Demonstrated successful protein removal and drug elution using a simple temperature change.

Conclusions:

  • A novel temperature-responsive spin column was successfully developed for serum sample preparation.
  • The all-aqueous eluent and temperature-dependent adsorption/elution offer a simplified approach to serum protein removal.
  • This method holds promise for improving the efficiency of serum drug analysis.